Long Non-Coding RNA Sequencing

Long Non-Coding RNA (lncRNA) Sequencing with Ribo-zero Treatment Service at Nucleome Informatics

Welcome to Nucleome Informatics’ Long Non-Coding RNA (lncRNA) Sequencing service, where we provide cutting-edge technologies and expert analysis to unlock the vast potential of non-coding RNAs. With over 10 years of extensive experience in genomics research, our state-of-the-art service encompasses advanced techniques, including Ribo-zero treatment, which enhances the accuracy and sensitivity of lncRNA sequencing.

Sample Requirement

To ensure successful lncRNA sequencing with Ribo-zero treatment, we require high-quality total RNA or enriched lncRNA samples. The following are the sample requirements for optimal library preparation:
  • Sample Quantity: A minimum of 200 ng of total RNA is needed for lncRNA sequencing library preparation.
  • Sample Purity: RNA samples must have an RNA Integrity Number (RIN) of 7 or higher to ensure minimal degradation and contamination.
  • Sample Concentration: RNA samples should have a concentration of at least 20 ng/μl.
  • Ribo-zero Treatment: Our lncRNA Sequencing service includes the application of Ribo-zero treatment during the library preparation step. Ribo-zero is a ribosomal RNA depletion technology that effectively removes rRNA molecules from the total RNA sample. By eliminating rRNA, we enrich the remaining RNA pool, including lncRNAs, small non-coding RNAs, and mRNA, which are crucial for studying gene expression regulation.

Library Preparation

Our expert team employs specialized library preparation methods to capture and enrich lncRNAs for accurate and comprehensive sequencing: RNA Isolation and Quality Control
  • We offer optional RNA isolation services to ensure high-quality RNA extraction.
  • Rigorous quality control measures are employed to assess RNA integrity and purity.
Ribo-zero Treatment
  • The Ribo-zero treatment is applied to selectively remove ribosomal RNA molecules from the total RNA sample.
lncRNA Enrichment
  • After Ribo-zero treatment, we perform lncRNA enrichment to selectively capture non-coding RNA molecules, including long non-coding RNAs, for focused sequencing.

Library Construction

  • Strand-specific library preparation to accurately determine the orientation of lncRNAs.
  • cDNA synthesis, adapter ligation, and amplification steps are optimized for robust library construction.

Library Quality Check

  • Stringent quality checks to evaluate library size distribution, concentration, and adapter ligation efficiency.

Sequencing on Illumina NovaSeq 6000

Our lncRNA Sequencing service utilizes the highly advanced Illumina NovaSeq 6000 platform, known for its superior sequencing capacity and cost-effectiveness.
  • Read Type: We offer paired-end (2×150 bp) sequencing for optimal coverage and accurate identification of lncRNAs.
  • Sequencing Depth: Our standard sequencing depth ensures comprehensive coverage, with the flexibility to customize based on your specific research needs.

Data Analysis

Our bioinformatics experts meticulously analyze the lncRNA sequencing data, providing a comprehensive report with actionable insights:
  • lncRNA Identification: Precise identification and annotation of lncRNAs from the sequencing data.
  • Differential Expression Analysis: Comparative analysis to identify differentially expressed lncRNAs across experimental conditions or sample groups.
  • Functional Annotation: Gene ontology and pathway analysis to uncover the biological functions of differentially expressed lncRNAs.
  • Interaction Network Analysis: Exploration of lncRNA-mRNA interactions to understand regulatory networks.

Importance of lncRNA Sequencing in Research

Plant Research Identifying lncRNAs involved in developmental processes, stress responses, and crop improvement.

Animal Research Exploring lncRNA-mediated gene regulation, embryonic development, and disease pathways.

Human Research Unraveling lncRNA signatures as potential biomarkers and therapeutic targets in various diseases, including cancer and neurodegenerative disorders.

Disease Research Understanding the roles of lncRNAs in disease pathogenesis, paving the way for novel diagnostic and therapeutic interventions. At Nucleome Informatics, we are dedicated to delivering accurate and insightful lncRNA sequencing results that fuel groundbreaking discoveries in genomics research. With our advanced technologies and experienced team, we aim to be your partner in advancing the understanding of lncRNA functions across diverse research fields.

Contact us today to discuss your lncRNA sequencing project and leverage our expertise in driving transformative discoveries.