Microbial Genome Sequencing

De Novo Microbial Genome Sequencing

De novo whole-genome sequencing involves assembling a genome without the use of a genomic reference and is often used to sequence novel microbial genomes. Illumina sequencers provide high coverage but shorter read lengths of unparalleled raw read accuracy. We also use PacBio RS II or Sequel for its higher read length and read depth for high-quality draft and complete microbial genome assemblies.

Microbial Whole-Genome Resequencing

Nucleome provides microbial whole-genome resequencing services to sequence the entire genome of a bacteria, virus, or other microbe, and comparing the sequence to that of a known reference for detecting low frequency mutations, finding key deletions and insertions, and discovering other genetic changes among microbial strains.